This component performs pairwise comparisons between fastas, given a multifasta as input for fastANI. It will split the multifasta into single fastas that will then be provided as a matrix. The output will be the all pairwise comparisons that pass the minimum of 50 aligned sequences with a default length of 200 bp.

Input/Output type

  • Input type: fasta
  • Output type: None


  • fragLen: Sets the minimum size of the fragment to be passed to

–fragLen argument of fastANI.

Published results

  • results/fast_ani/: A text file with the extension .out, which has all

the pairwise comparisons between sequences, reporting ANI.

Published reports


Default directives

  • fastAniMatrix:
    • container: flowcraft/fast_ani
    • version: 1.1.0-2
    • cpus: 20
    • memory: { 30.GB * task.attempt }